By Rudolf Pribil
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Extra info for Applied Complexometry
A freshly prepared solution i s colour l e s s b u t s l o w l y t u r n s y e l l o w owing t o d e c o m p o s i t i o n . The c o l o u r Sec. 1 ] Compiexometrie Indicators 43 change in the titration of calcium is only from pink to yellow, but some authors consider it an excellent indicator for calcium . 3. Dithizone Dithizone, diphenylthiocarbazone (XXIX), is one of the most sensitive but also most unselective reagents ever used in analytical chemistry. NH—NH—C 6 H 5 S=C \N=N—C 6H5 XXIX: Dithizone It has been used for colorimetrie determination of a number of metals.
3 3 ) . Svoboda [ l 3 l ] m i x t u r e as most u s e f u l . was p r o p o s e d by s u i t a b l e combin m i x t u r e of C a l c e i n recommends a 4 : 1 Sec. 2. 1 ] Complexometric Indicators 47 The pu  analysed various batches of the indicator from two suppliers by chromâtography. He recommends purification of a solution in aqueous ethanol (l + 2 ) of the indicator by column or batch chromatography on Y-alumina. 00025M concentration. The indicator is strongly debased by traces of fluoréscein, which cause residual fluorescence in the titration of calcium.
This has been proved by preparation of pure products [ΐ2θ]. The indicator 46 D e t e c t i o n of the T i t r a t i o n E n d - p o i n t [Ch. 2 i t s e l f has a g r e e n f l u o r e s c e n c e i n s o l u t i o n s of pH 3 - 1 1 · Above t h i s pH the f l u o r e s c e n c e weakens, and d i s a p p e a r s a t pH above 12. A s m a l l r e s i d u a l f l u o r e s c e n c e i s caused by t r a c e s of u n r e a c t e d I n s o l u t i o n s of pH above 12 the g r e e n f l u o r fluoré scein [ l 2 l ] .
Applied Complexometry by Rudolf Pribil