By David Evered
Main issue in regards to the environmental effects of the frequent use of insecticides has elevated, and proof of pesticide-resistant virus vectors have endured to emerge. This quantity provides a well timed survey of the mechanisms of plant resistance and examines present advancements in breeding for resistance, with specific emphasis on advances in genetic engineering which permit for the incorporation of viral genetic fabric into vegetation. Discusses the mechanisms of innate resistance in traces of tobacco, tomato, and cowpea; a variety of elements of brought about resistance, together with the characterization and roles of the pathogenesis-related proteins; antiviral components and their comparability with interferon; and cross-protection among plant virus lines. additionally provides a number of papers which evaluation the prestige of genetic engineering because it pertains to breeding resistant crops. between those are discussions of the capability use of plant viruses as gene vectors, gene coding for viral coat protein, satellite tv for pc RNA, and antisense RNA, and functional matters corresponding to the sturdiness of resistant crop vegetation within the box.
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Additional resources for Ciba Foundation Symposium 133 - Plant Resistance to Virus
Okada, and Y. Watanabe for collaborative work on TMV strains. References Atabekov JG, Dorokhov YuL 1984 Plant virus-specific transport function and resistance of plants to viruses. Adv Virus Res 29:313-364 Boccara M, Hamilton WDO, Baulcombe DC 1986 The organization and interviral homologies of genes at the 3' end of tobacco rattle virus RNA 1. EMBO (Eur Mol Biol Organ) J 5:223-229 Dawson WO, White JL 1978 Characterization of a temperature-sensitive mutant of tobacco mosaic virus deficient in synthesis of all RNA species.
It has the same specific infectivity and resistance to ribonuclease. Bol: If you inoculate the Arlington protoplasts with a mixture of the SB (CPMV) and DG (CPSMV) strains, does the cowpea severe mosaic virus permit replication of the cowpea mosaic virus RNA? Bruening: Are you asking whether the interference phenomenon holds up when we examine protoplasts? It is hard to do that experiment because, unlike the situation for the whole plant, the CPMV capsid alone, in the form of top component, is effective in protoplasts in interfering with DG.
The infectivity was defined as: Total no. of lesions X dilution factor Total no. 1 pg/ml) (2) The virus growth curve of the leaf discs was compared to that of protoplasts. Tomato plants were inoculated with Lsl and incubated for one day at 20-25 "C. Leaf discs were then prepared and incubated for an appropriate period (1-3 days) at either 22 or 32 "C. The virus growth curve of Lsl at 32 "C was found to show one-step growth, similar to that of Lsl in protoplasts, and the growth curve of Lsl at 22 "Cwas the same as that of L at either temperature.
Ciba Foundation Symposium 133 - Plant Resistance to Virus by David Evered