By Robert F. Vogt Jr., Gerald E. Marti, Vincent Zenger (auth.), Ute Resch-Genger (eds.)
The validation and standardization of fluorescence tools continues to be in its infancy in comparison to different favourite analytical and bioanalytical equipment. applicable caliber insurance criteria are despite the fact that a prerequisite for functions in hugely regulated fields resembling clinical diagnostics, drug improvement, or nutrients analysis.
For the 1st time, a group of well-known overseas specialists has documented the current prestige of caliber coverage in fluorescence measurements, and descriptions strategies for setting up criteria during this field.
This first of 2 volumes covers simple elements and diverse concepts equivalent to steady-state and time-resolved fluorometry, polarization thoughts, and fluorescent chemical sensors.
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Additional info for Standardization and Quality Assurance in Fluorescence Measurements I: Techniques
In some descriptive situations, semiquantitative terms such as “relatively dim” and “relatively bright” may be useful, but it is more precise to express the degree of proportionate difference between two measurements, such as twice or ten times as bright. Results expressed as MESF or ABC units should reference the standards or calibration system used to obtain the numeric results. Scientiﬁc reviewers should be alert for any misuse of calibrator values that do not have an authoritative or at least consensus basis, such as publication in peer-reviewed literature.
57 . . . . . . . Abstract The need for standardization in ﬂuorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of National Metrology Institutes (NMIs). Classes of ﬂuorescence standards are deﬁned, including instrument calibration standards for the determination and correction of instrument bias, application-speciﬁc standards based on commonly used ﬂuorescent labels, and instrument validation standards for periodic checks of instrument performance.
Keywords Calibration · Emission standards · Fluorescence intensity standards · Fluorescence standards · Quality assurance Abbreviations ABC Antibody binding capacity ASTM ASTM International BAM Federal Institute for Materials Research and Testing, Germany CRM Certiﬁed reference material EEM Excitation-emission matrix FDA United States Food and Drug Administration FL1 First ﬂuorescence channel of a ﬂow cytometer ISO International Organization for Standardization, Geneva IUPAC Union of Pure and Applied Chemistry LED Light emitting diode MESF Molecules of equivalent soluble ﬂuorophore NBS National Bureau of Standards, USA NIST National Institute of Standards and Technology, USA NMI National Metrology Institute NPL National Physical Laboratory, UK NRC National Research Council, Canada OLED Organic light emitting diode PTB Physikalisch-Technische Bundesanstalt, Germany R Measured reference signal S Measured ﬂuorescence signal, uncorrected Measured ﬂuorescence signal, corrected for detection system responsivity Scor SOP Standard operation procedure SRM® Standard Reference Material® 1 Introduction The use of ﬂuorescence continues to increase in the life and material sciences with many techniques having matured to a state where quantiﬁcation is desired [1–6].
Standardization and Quality Assurance in Fluorescence Measurements I: Techniques by Robert F. Vogt Jr., Gerald E. Marti, Vincent Zenger (auth.), Ute Resch-Genger (eds.)