By Igor S. Kulaev, Vladimir Vagabov, Tatiana Kulakovskaya
Now in a moment variation, Biochemistry of Inorganic Polyphosphates fills the necessity for an exhaustive source on inorganic polyphosphate metabolism. The authors describe the constitution and houses of those compounds and provides a comparative research of the most recent and conventional equipment in their extraction from cells. Distribution of polyphosphates in organisms, their localization in cells and tissues is usually defined. * finished presentation of inorganic polyphosphate metabolism * Follows polyphosphates in cells of organisms from diversified levels of evolution * offers tools for the research and learn of polyP-dependent enzymes * entire details on genetics, metabolism and biotechnology of polyphosphates * Textbook and reference paintings on all points of polyphosphates
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Extra info for The Biochemistry of Inorganic Polyphosphates
1996). The interaction with cations lays the basis for the 31 P NMR spectroscopic method, allowing one to distinguish between extracellular and intracellular pools of PolyPs. Ethylenediaminetetraacetic acid (EDTA) can be used to complex the divalent cations bound to PolyPs and to produce a new 31 P NMR shift. However, because the cell membrane is impermeabile to EDTA, only extracellular PolyPs is affected. This method was used successfully on a cell suspension of Propionibacterium sp. , 2002).
8 Concentration-dependence of the signal intensity ratio of the chemical shifts of core phosphate groups of linear PolyPs relative to the signal intensity of a standard Pi sample. PolyP samples with the following numbers of middle phosphate groups (n c ) in the molecules were used: (1) 204; (2) 115; (3) 58; (4) 35; (5) 11; (6) 3. , 1998). , 1998). , 1998). Synthetic linear PolyPs were used for this study after chromatographic puriﬁcation on Sephadex G-75. 9. 8. It can be seen that the contribution of the middle (n c ) groups to the total intensity of the P peaks decreases with an increase in the PolyP chain length.
1964). These researchers successively extracted the PolyPs present in the cells of Chlorella and other organisms with 8 % TCA in the cold (fraction A), then with a solution of NaOH, pH 9, in the cold (fraction B), and ﬁnally with a 2 N solution of KOH at 37 ◦ C for 18 h. e. one precipitated by neutralization with HClO4 in the presence of KClO4 (fraction C) and that which was not precipitated under these conditions (fraction D). The work of Miyachi showed that this mode of fractionation of Chlorella PolyPs yielded fractions which differed in their physiological activity and also apparently cellular location.
The Biochemistry of Inorganic Polyphosphates by Igor S. Kulaev, Vladimir Vagabov, Tatiana Kulakovskaya