Laura Soucek, Nicole M. Sodir's The Myc Gene: Methods and Protocols PDF

By Laura Soucek, Nicole M. Sodir

ISBN-10: 1627034285

ISBN-13: 9781627034289

ISBN-10: 1627034293

ISBN-13: 9781627034296

Myc controls a number of mobile capabilities, together with telephone proliferation, progress, differentiation and dying, either without delay and ultimately, via its modulation of downstream transcriptional courses. within the Myc Gene: equipment and Protocols, specialists within the box summarize the traditional and novel strategies that let the learning of Myc mechanism of motion in common and melanoma cells, in vitro and in vivo, in a single succinct handbook. Written within the hugely profitable equipment in Molecular Biology sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and key tips about troubleshooting and fending off recognized pitfalls.

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5 M EDTA; autoclave; add 1 ml 20 % (w/v) SDS; store at RT. 61. 1 % (w/v) SDS. 5 M EDTA; autoclave; add 1 ml 20 % (w/v) SDS; store at RT. 62. 05 % (w/v) SDS. 5 ml 20 % (w/v) SDS; store at RT. 63. 10× RNA gel running buffer: 200 mM MOPS [3-(N-morpholino)-propanesulfonic acid], 80 mM sodium acetate, 10 mM EDTA. 0 with 2 M NaOH; add H2O to 1,000 ml; store light-protected at 4 °C. 64. 03 % (w/v) bromophenol blue, 1× RNA gel running buffer, 1 % (w/v) Ficoll 400. 5 %), and 7 ml formamide; store in 1-ml aliquots at −20 °C.

4. Gently pull out the embryo with closed forceps under its neck. 5. Put the embryo onto a petri dish. Remove head, arms, and legs and transfer the body into a 50 ml tube. 6. Wash the body with 10 ml TS buffer (37 °C), shake briefly, decant the supernatant, and thoroughly homogenize the body with the round end of a spatula for 5 min. 7. Add 10 ml TR solution (37 °C), shake gently, incubate for 5 min at room temperature (RT), and then carefully pipette 5× up and down using a 10-ml wide-mouth pipette.

The highly oncogenic v-myc allele is derived from the chicken c-myc proto-oncogene by retroviral transduction [3, 4]. Subsequently, homologues of c-myc were identified in all vertebrate genomes. The protein product (Myc) of c-myc represents the key component of a transcriptional regulator network controlling the expression of a large fraction of all human genes [5, 6]. Myc is involved in the regulation of fundamental cellular processes like growth control, metabolism, proliferation, differentiation, and apoptosis.

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The Myc Gene: Methods and Protocols by Laura Soucek, Nicole M. Sodir

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